THE POTENTIOMETRIC UREA BIOSENSOR USING CHITOSAN MEMBRANE

被引：0
|
作者：
Mulyasuryani, Ani [1 ]
Roosdiana, Anna [1 ]
Srihardyastutie, Arie [1 ]
机构：
[1] Brawijaya Univ Jalan Veteran, Chem Dept, Malang, Indonesia
来源：
INDONESIAN JOURNAL OF CHEMISTRY | 2010年 / 10卷 / 02期
关键词：
biosensor; potentiometry; urea; chitosan membrane;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Potentiometric urea biosensor development is based on urea hydrolysis by urease resulted CO2. The biosensor is used chitosan membrane and the H3O+ electrode as a transducer. The research was studied of effecting pH and membrane thickness to the biosensor performance. The best biosensor performance resulted at pH 7.3 and membrane thickness of 0.2 mm. The biosensor has a Nerntian factor of 28.47 mV/decade; the concentration range is 0.1 up to 6.00 ppm; and the limit of detection is 0.073 ppm. The response time of this biosensor is 280 seconds, efficiency 32 samples and accuracy 94% up to 99%.
引用
收藏
页码：162 / 166
页数：5
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