CLONING AND CHARACTERIZATION OF THE RAD4 GENE OF SCHIZOSACCHAROMYCES-POMBE - A GENE SHOWING SHORT REGIONS OF SEQUENCE SIMILARITY TO THE HUMAN XRCC1 GENE

被引:64
|
作者
FENECH, M
CARR, AM
MURRAY, J
WATTS, FZ
LEHMANN, AR
机构
[1] UNIV SUSSEX,MRC,CELL MUTAT UNIT,BRIGHTON BN1 9RR,ENGLAND
[2] UNIV SUSSEX,SCH BIOL,MICROBIAL GENET LAB,BRIGHTON BN1 9RR,ENGLAND
基金
英国医学研究理事会;
关键词
D O I
10.1093/nar/19.24.6737
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The rad4.116 mutant of the fission yeast Schizosaccharomyces pombe is temperature-sensitive for growth, as well as being sensitive to the killing actions of both ultraviolet light and ionizing radiation. We have cloned the rad4 gene by complementation of the temperature sensitive phenotype of the rad4.116 mutant with a S. pombe gene bank. The rad4 gene fully complemented the UV sensitivity of the rad4.116 mutant. The gene is predicted to encode a protein of 579 amino acids with a basic tail, a possible zinc finger and a nuclear location signal. The amino terminal part of the predicted rad4 ORF contains two short regions of similarity to the C-terminal part of the human XRCC1 gene. Codon usage suggests that the gene is very poorly expressed, and this was confirmed by RNA studies. Gene disruption showed that the rad4 gene was essential for the mitotic growth of S.pombe.
引用
收藏
页码:6737 / 6741
页数:5
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