IMPROVED METHOD FOR COLIPHAGE DETECTION BASED ON BETA-GALACTOSIDASE INDUCTION

被引：7
|
作者：
IJZERMAN, MM
HAGEDORN, C
机构：
[1] Department of Crop and Soil Environmental Sciences, Virginia Polytechnic Institute, State University, Blacksburg, VA
来源：
JOURNAL OF VIROLOGICAL METHODS | 1992年 / 40卷 / 01期
关键词：
BETA-GALACTOSIDASE; COLIPHAGE; ISOPROPYL-BETA-D-THIOGALACTOSIDE; 2,3,5-TRIPHENYL TETRAZOLIUM CHLORIDE; 5-BROMO-4-CHLORO-3-INDOLYL-BETA-D-GALACTOPYRANOSIDE;
D O I：
10.1016/0166-0934(92)90004-W
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
An improved method for coliphage detection based on the induction of beta-galactosidase in Escherichia coli is described. Upon infection by coliphages, the cells are lysed and a stable indolyl product that is dark blue becomes visible within each plaque. The improved method is compared to the proposed coliphage detection procedure described in Standard Methods for the Examination of Water and Wastewater.
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页码：31 / 36
页数：6
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