COLOCALIZATION OF SUBSTANCE-P AND GABA IN RETINAL GANGLION-CELLS - A COMPUTER-ASSISTED VISUALIZATION

Ganglion cells in the albino rat retina were retrogradely labeled with the fluorescent dye, diamidino–yellow, from the superior colliculus. Preembedding and postembedding immunocytochemical techniques were employed in conjunction with computer–assisted image processing to visualize SP- and GABA-immunoreactivity. Examination of flatmount and sectioned retinas revealed that approximately 3% of the ganglion cells projecting to the contralateral superior colliculus exhibit SP–immunoreactivity. Moreover, these cells were found to comprise a subpopulation of the GABA–immunoreactive cells projecting to the rat tectum. © 1990, Cambridge University Press. All rights reserved.