2-STEP FREEZING OF THE RUMEN CILIATE PROTOZOAN ENTODINIUM-CAUDATUM

被引：7
|
作者：
KISIDAYOVA, S
机构：
[1] Slovak Academy of Sciences, Institute of Animal Physiology, Košice, 040 01 Košice
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 1995年 / 22卷 / 02期
关键词：
RUMEN CILIATE PROTOZOA; CRYOPRESERVATION; 2-STEP FREEZING; ONE-STEP FREEZING;
D O I：
10.1016/0167-7012(95)00003-4
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A procedure has been developed for the two-step deep freezing of the rumen ciliate protozoan Entodinium caudatum. The average recovery after 24 h of storage in liquid nitrogen (LN2) was 36% under the following conditions: a cell concentration 10(5)-10(6) ml(-1), 5 min equilibration at 25 degrees C with 4% DMSO (dimethylsulfoxide) as a cryoprotective agent and 45 min holding time at -25 degrees C. The samples were immersed into LN2 immediately after the holding phase of cooling. This two-step procedure significantly increases the survival of the rumen protozoan in comparison with one-step freezing. The protozoa preserve their growth ability after one year of storage in liquid nitrogen.
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页码：185 / 192
页数：8
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