INDUCTION OF NITRITE PRODUCTION IN MOUSE SPLEEN-CELLS BY IMMUNIZATION

被引：9
|
作者：
TAKAGI, K
NAKAGAMI, H
NAKANO, T
SUKETA, Y
机构：
[1] Department of Environment Biochemistry, School of Pharmaceutical Science, University of Shizuoka, Shizuoka
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1092卷 / 01期
关键词：
NITRITE; INTERFERON-GAMMA; IMMUNE RESPONSE; T-CELL REGULATION;
D O I：
10.1016/0167-4889(91)90172-T
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Changes of nitrite production in mouse spleen cells of in vitro secondary antibody response were investigated. Mouse spleen cells immunized with gamma globulin fraction of rat serum produced nitrite 3 days after in vitro challenging with the same antigen. Nitrite production of rabbit IgG-challenged spleen cells was found to about 2.9-times higher than that of spleen cells primed with the gamma globulin fraction of rat serum. Nitrite production in this system was completely suppressed by T cell depletion (99.7% inhibition). Furthermore, nitrite production in these cells significantly decreased by addition of anti-interferon gamma antibody (62.9% inhibition). These data indicate that nitrite production in antigen-immunized spleen cells is affected with the immunogenicity of an antigen and regulated by T cells, especially interferon (IFN) gamma.
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页码：15 / 20
页数：6
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