Electrophoretic Mobility of Red Blood Cells and Micronucleus Test in Exfoliated Buccal Cells as Stress Intensity Markers

The purpose of this study was to investigate the electrophoretic mobility of red blood cells (RBCs), cytomorphological and cytogenetical indices in exfoliated buccal epithelial cells of rats during the development of stress reaction. Experiments were carried out on 60 white non-pedigree pubescent rats weighing 180-220 g. For stress modeling, the animals of experimental groups received a single intraperitoneal injection of epinephrine hydrochloride solution (0.1 mg/kg) or injections for 3 days of the experiment. Both the single and the repeated adrenalin injections provoked a decrease in the level of the RBC electrophoretic mobility. With a single adrenalin injection, the changes in RBC electrophoretic mobility are not so abrupt, with the subsequent development of the adaptive response and reparation of cytogenetic damage. With repeated adrenalin injections, the changes in RBC electrophoretic mobility are abrupt, and the number of pathological epithelial cells increases.