Construction and analysis of alginate-based honey hydrogel as an ointment to heal of rat burn wound related infections

Developing a strategy for making the alginate base hydrogel components against burned wound infections could be promising for healing the mentioned wounds followed by elimination of the biofilm forming bacteria colonization. Construction of an alginate based hydrogel and evaluating healing activities of the mentioned component as local ointment were the main objectives of the current study. Following the collection of the honey from three different provinces of Iran, the components and structures of the collected materials were analyzed taking advantage of INSO-92 procedure subsequently, antibacterial effect of diluted three different kinds of honey against wild type bacterial species got evaluated via agar well diffusion method. An alginate base hydrogel was prepared by the use of calcium chloride as a linker between the alginate and honey functional groups. Then, component was structurally analyzed by Fourier Transform Infrared spectroscopy (FTIR). Afterward, under in vivo conditions, the healing activities of prepared ointment were studied in infected burned rat models. According to the antibacterial effect of the honeys, 75% diluted thymol based honeys collected from Damavand province were the most efficient ones. Furthermore, it was the healing activity of mentioned ointment was proven in vivo studies. The difference between 1600-1800 wave numbers in constructed alginate-based hydrogel alginate and honey because of C = 0 bond variations structurally confirmed proper construction of hydrogel. The hydrogel was the better healing activity in rats burned wound too. In conclusion the promising efficiency of alginate-based hydrogel in an elimination of bacterial infections was confirmed as the main aim of the current survey.