DECREASE OF PLATINUM ACCUMULATION IN A CISPLATIN-RESISTANT CHINESE-HAMSTER OVARY CELL-LINE

被引：0

作者：

HUANG, HM ^{[1
]}

CHANG, HW ^{[1
]}

HSIEH, HG ^{[1
]}

TSAI, LC ^{[1
]}

机构：

[1] VET GEN HOSP, DEPT MED RES, TAIPEI, TAIWAN

来源：

BULLETIN OF THE INSTITUTE OF ZOOLOGY ACADEMIA SINICA | 1992年 / 31卷 / 03期

关键词：

CISPLATIN RESISTANCE; CISPLATIN ACCUMULATION; CISPLATIN-DNA MONOCLONAL ANTIBODY;

D O I：

暂无

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

Cisplatin-resistant cells, Pt-r6, were developed through the chronic exposure of Chinese hamster ovary (CHO-K1) cells to increasing concentrations of cisplatin. The LD50 of clonogenic survival after a 2-h cisplatin treatment was 16.3 and 7-mu-M for resistant Pt-r6 and parental CHO-K1 cells, respectively. An analysis of cellular DNA adducts by cisplatin-DNA monoclonal antibodies using the ELISA or flow cytometric methods of adduct-antibody conjugation with fluorescent dye revealed that more adducts were induced in CHO-K1 cells than in Pt-r6 cells. Moreover, CHO-K1 cells also accumulated more cisplatin than Pt-r6 cells, as determined by inductively coupled plasma-atomic emission spectrometry. Thus, resistance to cisplatin was clearly correlated with decreased amounts of platinum and cisplatin-DNA adducts in the resistant cells as compared to the sensitive cells. These results suggest that the reduced accumulation of cisplatin may be involved in the cisplatin resistance of Pt-r6 cells.

引用

页码：163 / 168

页数：6

共 50 条

[41] THE EFFECT OF HYPOXIA ON CYTOTOXICITY, ACCUMULATION AND DNA-BINDING OF CISPLATIN IN CHINESE-HAMSTER OVARY CELLS
MATTHEWS, JB
ADOMAT, H
SKOV, KA
ANTI-CANCER DRUGS, 1993, 4 (04) : 463 - 470
[42] DIAMINOPURINE RESISTANT MUTANTS OF CHINESE-HAMSTER OVARY CELLS
PIPKORN, J
TOKITO, M
TAYLOR, MW
IN VITRO-JOURNAL OF THE TISSUE CULTURE ASSOCIATION, 1976, 12 (04): : 307 - 308
[43] CYTOTOXICITY AND METABOLISM OF PREDNIMUSTINE, CHLORAMBUCIL AND PREDNISOLONE IN A CHINESE-HAMSTER CELL-LINE
HARTLEYASP, B
GUNNARSSON, PO
LILJEKVIST, J
CANCER CHEMOTHERAPY AND PHARMACOLOGY, 1986, 16 (02) : 85 - 90
[44] ISOLATION AND CHARACTERIZATION OF A CHINESE-HAMSTER CELL-LINE DEFECTIVE IN METABOLISM OF ADENINE
CRAWFORD, CR
BRYANT, RE
FEDERATION PROCEEDINGS, 1980, 39 (06) : 1745 - 1745
[45] THE R-BANDING PATTERN OF THE CHINESE-HAMSTER DON CELL-LINE
MARTINEZ, A
PASANTES, JJ
GONZALEZ, A
GOYANES, V
MENDEZ, J
GENETICA, 1989, 78 (01) : 51 - 55
[46] SEQUENCE SPECIFICITY OF CYTOSINE METHYLATION IN THE DNA OF THE CHINESE-HAMSTER OVARY (CHO-K1) CELL-LINE
WOODCOCK, DM
CROWTHER, PJ
SIMMONS, DL
COOPER, IA
BIOCHIMICA ET BIOPHYSICA ACTA, 1984, 783 (03) : 227 - 233
[47] LONG-TERM MULTIPLICATION OF THE CHINESE-HAMSTER OVARY (CHO) CELL-LINE IN A SERUM-FREE MEDIUM
GASSER, F
MULSANT, P
GILLOIS, M
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY, 1985, 21 (10): : 588 - 592
[48] STRUCTURE OF THE CARBOHYDRATE MOIETY OF HUMAN INTERFERON-BETA SECRETED BY A RECOMBINANT CHINESE-HAMSTER OVARY CELL-LINE
CONRADT, HS
EGGE, H
PETERKATALINIC, J
REISER, W
SIKLOSI, T
SCHAPER, K
JOURNAL OF BIOLOGICAL CHEMISTRY, 1987, 262 (30) : 14600 - 14605
[49] ENDOGENOUS ORIGIN OF DEFECTIVE RETROVIRUS-LIKE PARTICLES FROM A RECOMBINANT CHINESE-HAMSTER OVARY CELL-LINE
ANDERSON, KP
LOW, MAL
LIE, YS
KELLER, GA
DINOWITZ, M
VIROLOGY, 1991, 181 (01) : 305 - 311
[50] A TEMPERATURE-SENSITIVE DNA-SYNTHESIS MUTANT ISOLATED FROM THE CHINESE-HAMSTER OVARY CELL-LINE
MCCRACKEN, AA
SOMATIC CELL GENETICS, 1982, 8 (02): : 179 - 195

← 1 2 3 4 5 →