IN-VITRO RNA EDITING-LIKE ACTIVITY IN A MITOCHONDRIAL EXTRACT FROM LEISHMANIA-TARENTOLAE

被引:30
|
作者
FRECH, GC
BAKALARA, N
SIMPSON, L
SIMPSON, AM
机构
[1] UNIV CALIF LOS ANGELES,HOWARD HUGHES MED INST,LOS ANGELES,CA 90024
[2] UNIV CALIF LOS ANGELES,DEPT MED MICROBIOL & IMMUNOL,LOS ANGELES,CA 90024
来源
EMBO JOURNAL | 1995年 / 14卷 / 01期
关键词
LEISHMANIA; MITOCHONDRIA; RNA EDITING; TUTASE;
D O I
10.1002/j.1460-2075.1995.tb06988.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A mitochondrial extract from Leishmania tarentolae directs the incorporation of uridylate (U) residues within the pre-edited domain of synthetic cytochrome b (CYb) and NADH dehydrogenase subunit 7 mRNA. This has several characteristics of an in vitro RNA editing activity, but no direct evidence for involvement of guide RNAs was obtained. Inhibition by micrococcal nuclease suggests a requirement for some type of endogenous RNA. The limitation of internal U-incorporation to the pre-edited region in the CYb mRNA and the inhibition by deletion or substitution of both mRNA anchor sequences for CYb gRNA-I and -II could be consistent either with a gRNA-mediated process or a secondary structure-mediated process. A low level of incorporation of [alpha-P-32]CTP occurs at the same sites as UTP. Internal U-incorporation activity is selectively inhibited by heterologous RNAs, suggesting an involvement of low affinity RNA-binding proteins which can be competed by the added RNA.
引用
收藏
页码:178 / 187
页数:10
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