SPLICING OF A CIRCULAR YEAST PRE-MESSENGER-RNA IN-VITRO

被引：11
|
作者：
SCHINDEWOLF, CA [1 ]
DOMDEY, H [1 ]
机构：
[1] UNIV MUNICH,GENZENTRUM,INST BIOCHEM,D-81375 MUNICH,GERMANY
来源：
NUCLEIC ACIDS RESEARCH | 1995年 / 23卷 / 07期
关键词：
D O I：
10.1093/nar/23.7.1133
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have tested the fate of a circularized synthetic pre-mRNA transcript in a whole cell splicing extract of Saccharomyces cerevisiae. Our results demonstrate that this circular precursor RNA is able to induce spliceosome formation in vitro and that the products of the following splicing reaction are the lariat-shaped intron, and a mature circular mRNA. Thus, it would appear that free 5' and/or 3' ends are not obligatory for a splicing reaction to occur, although we find its efficiency to be strongly influenced by the presence or lack of free ends. To our knowledge, this is the first demonstration that a circular pre-mRNA molecule is recognized as a suitable substrate by an eukaryotic mRNA splicing apparatus.
引用
收藏
页码：1133 / 1139
页数：7
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