CLONING, PHARMACOLOGICAL CHARACTERISTICS AND EXPRESSION PATTERN OF THE RAT GABA-A RECEPTOR ALPHA-4 SUBUNIT

被引：253
|
作者：
WISDEN, W [1 ]
HERB, A [1 ]
WIELAND, H [1 ]
KEINANEN, K [1 ]
LUDDENS, H [1 ]
SEEBURG, PH [1 ]
机构：
[1] CTR MOLEC BIOL,MOLEC NEUROENDOCRINOL LAB,NEUENHEIMER FELD 282,W-6900 HEIDELBERG,GERMANY
来源：
FEBS LETTERS | 1991年 / 289卷 / 02期
关键词：
GABA-A; BENZODIAZEPINE RECEPTOR; INSITU HYBRIDIZATION; THALAMUS; ALPHA-SUBUNIT HETEROGENEITY;
D O I：
10.1016/0014-5793(91)81076-K
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A cDNA of rat brain encoding the GABA(A) receptor alpha-4 subunit has been cloned. Recombinant receptors composed of alpha-4, beta-2 and gamma-2 subunits bind with high affinity the GABA agonist [H-3]muscimol and the benzodiazepine 'alcohol antagonist' [H-3]Ro 15-4513, but fail to bind benzodiazepine agonists. The alpha-4 subunit is expressed mainly in the thalamus, as assessed by in situ hybridization histochemistry, and may participate in a major population of thalamic GABA(A) receptors. The alpha-4, mRNA is found at lower levels in cortex and caudate putamen, and is rare in cerebellum.
引用
收藏
页码：227 / 230
页数：4
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