ACTIVATION-ASSOCIATED MARKER PROTEINS - PEPTIDE-MAPPING AND THEIR EXPRESSION IN MACROPHAGE CELL-LINES

被引：3

作者：

PINSON, DM

PHILLIPS, TA

PACE, JL

MACKAY, RJ

RUSSELL, SW

机构：

[1] UNIV KANSAS,MED CTR,CTR CANC,WILKINSON LAB CANC RES,1008 WAHL HALL W,39TH & RAINBOW BLVD,KANSAS CITY,KS 66103

[2] UNIV KANSAS,MED CTR,DEPT PATHOL ONCOL,KANSAS CITY,KS 66103

[3] UNIV KANSAS,MED CTR,DEPT MICROBIOL,KANSAS CITY,KS 66103

[4] UNIV KANSAS,MED CTR,DEPT MOLEC GENET,KANSAS CITY,KS 66103

[5] UNIV KANSAS,MED CTR,DEPT IMMUNOL,KANSAS CITY,KS 66103

[6] UNIV FLORIDA,COLL VET SCI,DEPT LARGE ANIM CLIN SCI,GAINESVILLE,FL 32610

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 176卷 / 02期

关键词：

D O I：

10.1016/S0006-291X(05)80268-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The activation-associated markers, p47b and p71/73, have been recognized as minor proteins in peritoneal and bone marrow culture-derived macrophages activated for tumor cell killing. Proteins with identical characteristics of migration on 2-dimensional gels and comparable Cleveland peptide maps are described here in macrophage cell lines that could be activated for tumor cell killing (J774A.1, RAW 264, UNC-2). Macrophage cell lines that could not be activated (P388D1 and PU5-1.8) did not express the markers. The expression of these markers in activatable macrophage cell lines strengthens their association with the activation process and provides a bulk source of the proteins for purification studies. © 1991 Academic Press, Inc.

引用

页码：882 / 886

页数：5

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