In vitro Enzymatic Assays of Histone Decrotonylation on Recombinant Histones

被引：1
|
作者：
Fellows, Rachel [1 ]
Varga-Weisz, Patrick [1 ,2 ]
机构：
[1] Babraham Inst, Cambridge, England
[2] Univ Essex, Sch Biol Sci, Colchester, Essex, England
来源：
BIO-PROTOCOL | 2018年 / 8卷 / 14期
基金：
英国生物技术与生命科学研究理事会;
关键词：
Crotonylation; Acetylation; Butyrylation; Histone deacetylase; Enzyme; Kinetic; Chromatin;
D O I：
10.21769/BioProtoc.2924
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Class I histone deacetylases (HDACs) are efficient histone decrotonylases, broadening the enzymatic spectrum of these important (epi-)genome regulators and drug targets. Here, we describe an in vitro approach to assaying class I HDACs with different acyl-histone substrates, including crotonylated histones and expand this to examine the effect of inhibitors and estimate kinetic constants.
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页数：20
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