NONCOVALENT BINDING OF A MITOMYCIN-C METABOLITE, 2,7-DIAMINOMITOSENE, TO DUPLEX DNA

被引：6
|
作者：
PETERSON, DM
FISHER, J
BEALL, HD
ROSS, D
机构：
[1] UNIV COLORADO, HLTH SCI CTR, SCH PHARM, DENVER, CO 80262 USA
[2] UNIV COLORADO, HLTH SCI CTR, CTR CANC, DENVER, CO 80262 USA
来源：
CANCER LETTERS | 1995年 / 90卷 / 02期
关键词：
MITOMYCIN C; 2,7-DIAMINOMITOSENE (DAM); BIOREDUCTIVE ACTIVATION; INTERCALATION; NONCOVALENT INTERACTION WITH DNA; DT-DIAPHORASE;
D O I：
10.1016/0304-3835(95)03694-R
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The major metabolite of mitomycin C, 2,7-diaminomitosene (DAM), interacts noncovalently with DNA. This was supported by ultraviolet-visible spectrum changes upon mixing with DNA and ethidium bromide displacement from DNA, measured as fluorescence changes. Moreover, DAM bound to DNA sufficiently strongly to hold DNA in a double stranded conformation under denaturing gel electrophoresis conditions commonly used to measure mitomycin C cross-links. These data show that generation of DAM and interaction with DNA represent a potential additional mechanism of DNA damage induced by mitomycin C.
引用
收藏
页码：133 / 138
页数：6
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