DETECTION OF NON-CULTURABLE VIBRIO-CHOLERAE O139, BY PCR AND FLUORESCENT-ANTIBODY METHODS, IN LABORATORY MICROCOSMS

被引：4
|
作者：
ISLAM, MS
ALAM, MJ
MIAH, MA
FELSENSTEIN, A
SACK, RB
机构：
[1] Environmental Microbiology Laboratory, Laboratory Sciences Division, International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR, B), Dhaka, 1000
来源：
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1995年 / 11卷 / 05期
关键词：
MICROCOSM; NON-CULTURABLE; PCR; VIBRIO CHOLERAE O139;
D O I：
10.1007/BF00286382
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Non-culturable Vibrio cholerae O139 was detected in microcosms by PCR and fluorescent-antibody (FA) techniques. When survival of V. cholerae O139 in microcosms was assessed by viable counting on culture media, the vibrio became non-culturable after 44 days and remained non-culturable for an additional 7 weeks.
引用
收藏
页码：597 / 598
页数：2
相关论文
共 50 条
  • [41] NATIONWIDE PREVALENCE OF THE NEW EPIDEMIC STRAIN OF VIBRIO-CHOLERAE O139 BENGAL IN INDIA
    GARG, S
    SAHA, PK
    RAMAMURTHY, T
    DEB, BC
    NAIR, GB
    SHIMADA, T
    TAKEDA, Y
    JOURNAL OF INFECTION, 1993, 27 (01) : 108 - 109
  • [42] ENUMERATION OF VIBRIO-CHOLERAE O1 IN BANGLADESH WATERS BY FLUORESCENT-ANTIBODY DIRECT VIABLE COUNT
    BRAYTON, PR
    TAMPLIN, ML
    HUQ, A
    COLWELL, RR
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1987, 53 (12) : 2862 - 2865
  • [43] TCPA PILIN SEQUENCES AND COLONIZATION REQUIREMENTS FOR O1 AND O139 VIBRIO-CHOLERAE
    RHINE, JA
    TAYLOR, RK
    MOLECULAR MICROBIOLOGY, 1994, 13 (06) : 1013 - 1020
  • [44] CHARACTERIZATION OF PHAGE PHI-O139, A VIBRIO-CHOLERAE O139 TEMPERATE BACTERIOPHAGE WITH COHESIVE DNA TERMINI
    PAJNI, S
    CHOWDHURY, NR
    GHOSH, A
    KAR, S
    GHOSH, RK
    FEMS MICROBIOLOGY LETTERS, 1995, 131 (01) : 69 - 74
  • [45] A RETROSPECTIVE ANALYSIS OF THE MADRAS EPIDEMIC OF NON-O1 VIBRIO-CHOLERAE NEW SEROGROUP O139 BENGAL
    DHAMODARAN, S
    ANANTHAN, S
    KUGANANTHAM, P
    INDIAN JOURNAL OF MEDICAL RESEARCH, 1995, 101 : 94 - 97
  • [46] GENOMIC FINGERPRINTING OF VIBRIO-CHOLERAE O139 FROM GERMANY AND SOUTH-ASIA IN COMPARISON WITH STRAINS OF VIBRIO-CHOLERAE O1 AND OTHER SEROGROUPS
    PRAGER, R
    STRECKEL, W
    STEPHAN, R
    BOCKEMUHL, J
    SHIMADA, T
    TSCHAPE, H
    MEDICAL MICROBIOLOGY LETTERS, 1994, 3 (05): : 219 - 227
  • [47] DETECTION OF VIBRIO-CHOLERAE O1 IN THE AQUATIC ENVIRONMENT BY FLUORESCENT-MONOCLONAL ANTIBODY AND CULTURE METHODS
    HUQ, A
    COLWELL, RR
    RAHMAN, R
    ALI, A
    CHOWDHURY, MAR
    PARVEEN, S
    SACK, DA
    RUSSEKCOHEN, E
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1990, 56 (08) : 2370 - 2373
  • [48] PRODUCTION, CHARACTERIZATION, AND APPLICATION OF MONOCLONAL-ANTIBODIES TO VIBRIO-CHOLERAE O139 SYNONYM BENGAL
    QADRI, F
    AZIM, T
    CHOWDHURY, A
    HOSSAIN, J
    SACK, RB
    ALBERT, MJ
    CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 1994, 1 (01) : 51 - 54
  • [49] NEW STRAINS OF VIBRIO-CHOLERAE O139 IN INDIA AND BANGLADESH - LESSONS FROM THE RECENT EPIDEMICS
    RABBANI, GH
    MAHALANABIS, D
    JOURNAL OF DIARRHOEAL DISEASES RESEARCH, 1993, 11 (02): : 63 - 66
  • [50] EVALUATION OF THE MONOCLONAL ANTIBODY-BASED KIT BENGAL SMART FOR RAPID DETECTION OF VIBRIO-CHOLERAE O139 SYNONYM BENGAL IN STOOL SAMPLES
    QADRI, F
    HASAN, JAK
    HOSSAIN, J
    CHOWDHURY, A
    BEGUM, YA
    AZIM, T
    LOOMIS, L
    SACK, RB
    ALBERT, MJ
    JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (03) : 732 - 734
12345