ENRICHMENT OF EICOSAPENTAENOIC ACID AND DOCOSAHEXAENOIC ACID-ESTERS FROM ESTERIFIED FISH OIL BY PROGRAMMED EXTRACTION ELUTION WITH SUPERCRITICAL CARBON-DIOXIDE

被引：26
|
作者：
HIGASHIDATE, S
YAMAUCHI, Y
SAITO, M
机构：
[1] JASCO, Japan Spectroscopic Co., Ltd., Hachioji City, Tokyo, 192
来源：
JOURNAL OF CHROMATOGRAPHY | 1990年 / 515卷
关键词：
D O I：
10.1016/S0021-9673(01)89324-9
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Methyl esters of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in esterified fish oil were extracted by supercritical fluid extraction with carbon dioxide and directly introduced into a silica gel column coated with silver nitrate. Supercritical fluid chromatography with carbon dioxide was then performed by changing stepwise the pressure of the column outlet. The EPA and DHA methyl esters thus separated were fractionated by reducing the pressure of column effluent to atmospheric. In this way, EPA and DHA methyl esters were enriched from 12% to 93% and from 13% to 82%, respectively. © 1990.
引用
收藏
页码：295 / 303
页数：9
相关论文
共 50 条
  • [11] DIFFUSION OF FATTY-ACID ESTERS IN SUPERCRITICAL CARBON-DIOXIDE
    LIONG, KK
    WELLS, PA
    FOSTER, NR
    INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, 1992, 31 (01) : 390 - 399
  • [12] Solubility of fish oil fatty acid ethyl esters in sub and supercritical carbon dioxide
    Staby, Arne, 1600, (70):
  • [13] Concentration of eicosapentaenoic acid and docosahexaenoic acid from fish oil by hydrolysis and urea complexation
    Gámez-Meza, N
    Noriega-Rodríguez, JA
    Medina-Juárez, LA
    Ortega-García, J
    Monroy-Rivera, J
    Toro-Vázquez, FJ
    García, HS
    Angulo-Guerrero, O
    FOOD RESEARCH INTERNATIONAL, 2003, 36 (07) : 721 - 727
  • [14] Eicosapentaenoic Acid Extraction from Nannochloropsis gaditana Using Carbon Dioxide at Supercritical Conditions
    Molino, Antonio
    Martino, Maria
    Larocca, Vincenzo
    Di Sanzo, Giuseppe
    Spagnoletta, Anna
    Marino, Tiziana
    Karatza, Despina
    Iovine, Angela
    Mehariya, Sanjeet
    Musmarra, Dino
    MARINE DRUGS, 2019, 17 (02):
  • [15] Divergent effects of eicosapentaenoic and docosahexaenoic acid ethyl esters, and fish oil on hepatic fatty acid oxidation in the rat
    Hong, DD
    Takahashi, Y
    Kushiro, M
    Ide, T
    BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 2003, 1635 (01): : 29 - 36
  • [16] SOLUBILITY AND PARTITION-COEFFICIENTS OF FISH-OIL ESTERS IN SUPERCRITICAL CARBON-DIOXIDE
    STABY, A
    BORCHJENSEN, C
    MOLLERUP, J
    JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 1994, 27 (01) : 132 - 134
  • [17] Separation of fatty acid esters from cholesterol in esterified natural and synthetic mixtures by supercritical carbon dioxide
    Yeh, An-I., 1600, (68):
  • [18] Assessment of lipid composition and eicosapentaenoic acid/docosahexaenoic acid bioavailability in fish oil obtained through different enrichment methods
    Song, Rongzhen
    Li, Wen
    Deng, Shanggui
    Zhao, Yueliang
    Tao, Ningping
    FRONTIERS IN NUTRITION, 2023, 10
  • [19] ABSORPTION OF EICOSAPENTAENOIC ACID AND DOCOSAHEXAENOIC ACID FROM FISH OIL TRIACYLGLYCEROLS OR FISH OIL ETHYL-ESTERS CO-INGESTED WITH A HIGH-FAT MEAL
    LAWSON, LD
    HUGHES, BG
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 156 (02) : 960 - 963
  • [20] SUPERCRITICAL CARBON-DIOXIDE EXTRACTION OF TERPENES FROM ORANGE ESSENTIAL OIL
    TEMELLI, F
    BRADDOCK, RJ
    CHEN, CS
    NAGY, S
    ACS SYMPOSIUM SERIES, 1988, 366 : 109 - 126
12345