EXPRESSION AND PURIFICATION OF FUSION PROTEINS CONTAINING NA+, K+-ATPASE ALPHA-SUBUNIT FRAGMENTS THAT ARE LOCATED NEAR THE MEMBRANE

被引：0
|
作者：
LARIN, DI
SHAKHPARONOV, MI
ORTIZ, E
KOSTINA, MB
MODYANOV, NN
机构：
来源：
BIOLOGICHESKIE MEMBRANY | 1994年 / 11卷 / 05期
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D O I：
暂无
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
A new method is described for the expression and purification of hybrid proteins consisting of human tumor necrosis factor and the alpha-subunit fragments of pig Na+, K+-ATPase that are located near or inside the plasma membrane. Six fusion proteins were obtained to be used in producing monoclonal antibodies against the ATPase fragments.
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页码：469 / 475
页数：7
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