SUPPRESSION OF FIBROSARCOMA METASTASIS BY ELEVATED EXPRESSION OF MANGANESE SUPEROXIDE-DISMUTASE

A mouse fibrosarcoma cell line (FSa-II), which exhibits low endogenous levels of manganese superoxide dismutase, was transfected with a human manganese superoxide dismutase complementary DNA. Fifty clones were screened for manganese superoxide dismutase activity by the superoxide dismutase activity gel assay. Activity of the positive clones was measured by the nitro blue tetrazolium reduction assay in the presence of cyanide, Three cell lines exhibiting a range of activity were chosen to be transplanted into syngeneic mice. The results indicated that the metastasis rate for all transfected cells was significantly less than that of control cells.