STEREOISOMERIC INVERSION OF (25R)-3-ALPHA,7-ALPHA,12-ALPHA-TRIHYDROXY-5-BETA-CHOLESTANOIC AND (25S)-3-ALPHA,7-ALPHA,12-ALPHA-TRIHYDROXY-5-BETA-CHOLESTANOIC ACIDS IN RAT-LIVER PEROXISOME

被引：0
|
作者：
IKEGAWA, S
GOTO, T
WATANABE, H
GOTO, J
机构：
来源：
BIOLOGICAL & PHARMACEUTICAL BULLETIN | 1995年 / 18卷 / 07期
关键词：
5-BETA-CHOLESTANOIC ACID; EPIMERIZATION; PEROXISOME; APCI-MS; NICI DETECTION; GC/MS; LC/MS;
D O I：
暂无
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
The stereoisomeric inversion of (25R)- and (25S)-3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestanoic acid (THCA) in rat liver peroxisome was studied. After incubation of an isomer of THCA-CoA thioester with a peroxisomal fraction, 5 beta-cholestanoic acids were extracted and optical antipodes were separated and determined by LC/APCI-MS. The transformation of (24E)-3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholest-24-en-26-oic acid (Delta(24)-THCA) formed by acyl-CoA oxidase was also analyzed by GC/NICI-MS. Rapid enzymatic epimerization from either direction was observed prior to biotransformation into (24E)-Delta(24)-THCA.
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页码：1027 / 1029
页数：3
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