DIRECT DETECTION OF CIRCULATING FREE-RADICALS IN THE RAT USING ELECTRON-SPIN-RESONANCE SPECTROMETRY

被引：30
|
作者：
WANG, XY
LIU, JK
YOKOI, I
KOHNO, M
MORI, A
机构：
[1] OKAYAMA UNIV,SCH MED,INST NEUROBIOL,DEPT NEUROCHEM,2-5-1 SHIKATACHO,OKAYAMA 700,JAPAN
[2] JEOL LTD,CTR APPLICAT & RES,ESR APPLICAT LAB,TOKYO 196,JAPAN
来源：
FREE RADICAL BIOLOGY AND MEDICINE | 1992年 / 12卷 / 02期
关键词：
ELECTRON SPIN RESONANCE; ASCORBIC ACID; TRANSITION METALS; BLOOD; IRON-OVERLOAD; FREE RADICALS;
D O I：
10.1016/0891-5849(92)90005-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We developed a new technique for directly observing in vivo free radical formation in the circulating blood of living rats using electron spin resonance (ESR) spectrometry without any labeling or trapping agents. It was found that a doublet peak spectrum was obtained following ferric citrate and ascorbic acid injection. The signals were confirmed in different ways to be due to ascorbic acid radicals. These results provide evidence to support the involvement of free radical intermediates in iron-ascorbic acid reactions, and further confirm the suggested mechanism of both the adverse and protective effects of ascorbic acid in biological systems. Furthermore, this method of direct observation is a new application of ESR spectrometry to living animals.
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页码：121 / 126
页数：6
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