PILI OF AN AEROMONAS-HYDROPHILA STRAIN AS A POSSIBLE COLONIZATION FACTOR

被引：33
|
作者：
HOKAMA, A
HONMA, Y
NAKASONE, N
机构：
[1] Department of Bacteriology, University of the Ryukyus School of Medicine, Nishihara, Okinawa
来源：
MICROBIOLOGY AND IMMUNOLOGY | 1990年 / 34卷 / 11期
关键词：
D O I：
10.1111/j.1348-0421.1990.tb01069.x
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Aeromonas hydrophila Ae6 had 2 morphologically distinctive kinds of pili. One appeared rigid and straight with a diameter of 9 nm (R-pili). The other appeared wavy and flexible with a diameter of 7 nm (W-pili). W-pili were very few on the cell as compared with R-pili. In this study, W-pili were purified and characterized. The pili consisted of a subunit protein with a molecular weight of 21 kDa as estimated by SDS-PAGE. There was no immunological cross-reaction between W-pili and other cellular components. The strain Ae6 and its purified W-pili adhered to human and rabbit intestine and agglutinated human and rabbit erythrocytes. Organisms pretreated with the Fab fraction of anti-pilus antibody failed to adhere to the intestine. Pretreatment of intestine with purified W-pili blocked adherence of the organisms to the intestine. These results suggest that the W-pili are the colonization factor of A. hydrophila Ae6.
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页码：901 / 915
页数：15
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