HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF URINARY OLIGOSACCHARIDES IN THE DIAGNOSIS OF GLYCOPROTEIN DEGRADATION DISORDERS

被引：11
|
作者：
HOMMES, FA
VARGHESE, M
机构：
[1] Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta
来源：
CLINICA CHIMICA ACTA | 1991年 / 203卷 / 2-3期
关键词：
HPLC; URINARY OLIGOSACCHARIDES; GLYCOPROTEIN DEGRADATION;
D O I：
10.1016/0009-8981(91)90293-L
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Urinary oligosaccharides can be separated by high-performance anion-exchange chromatography using a Dionex CarboPac PA1 column, elution with aqueous sodium hydroxide and sodium acetate solutions and detection by pulsed amperometry. Each of the urines of patients with glycoprotein degradation disorders yielded a pattern of oligosaccharide excretion unique for that disorder, facilitating an unambiguous diagnosis. The method is sensitive (10-mu-l of urine required) and fast (40 min).
引用
收藏
页码：211 / 224
页数：14
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