GENOMIC MHC HAPLOTYPING OF MUTANT-CELL LINES USING A NOVEL MARKER

被引：0
|
作者：
DEGLIESPOSTI, MA
WILLIAMSON, JF
TAY, GK
SALMON, B
JOSEPH, GA
MORLEY, AA
DAWKINS, RL
机构：
[1] ROYAL PERTH HOSP,DEPT CLIN IMMUNOL,GPO BOX X2213,PERTH,WA 6001,AUSTRALIA
[2] SIR CHARLES GAIRDNER HOSP,NEDLANDS,WA 6009,AUSTRALIA
[3] UNIV WESTERN AUSTRALIA,PERTH,WA,AUSTRALIA
[4] FLINDERS UNIV S AUSTRALIA,MED CTR,DEPT HAEMATOL,BEDFORD PK,SA 5042,AUSTRALIA
来源：
EUROPEAN JOURNAL OF IMMUNOGENETICS | 1993年 / 20卷 / 05期
关键词：
D O I：
10.1111/j.1744-313X.1993.tb00156.x
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The aim of this study was to characterize MHC mutant cell lines by studying haplospecific markers within the MHC and specifically in the 250 kilobase (kb) region between the HLA B and TNF loci. This region has been difficult to define because of the lack of appropriate markers. Spontaneous MHC mutants were isolated after immunoselection with an anti-HLA A2 monoclonal antibody and complement. Ten mutants were characterized using serological or allelic and genomic DNA markers within the HLA A to HLA DQ region ot the MHC. Most mutants lost at least the 3 megabases of DNA from HLA A to HLA DQ viz the whole haplotype carrying HLA A2. Variants which have lost either HLA A alone or HLA A and HLA B were also found. The results show that it is possible to map the extent of the deletion between HLA B and TNF. Haplospecific scanning patterns for the CL region appear particularly useful.
引用
收藏
页码：373 / 380
页数：8
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