A NEW METHOD OF HUMAN CARDIAC TROPONIN-I AND TROPONIN-T PURIFICATION

被引：0
|
作者：
KATRUKHA, AG
BEREZNIKOVA, AV
ESAKOVA, TV
FILATOV, VL
BULARGINA, TV
GUSEV, NB
机构：
[1] MOSCOW MV LOMONOSOV STATE UNIV,SCH BIOL,DEPT BIOCHEM,MOSCOW 119899,RUSSIA
[2] MOSCOW MV LOMONOSOV STATE UNIV,RUSSIAN CTR MOLEC DIAGNOST & MED CARE,MOSCOW 119899,RUSSIA
[3] MOSCOW MV LOMONOSOV STATE UNIV,DEPT BIOORGAN CHEM,MOSCOW 119899,RUSSIA
来源：
BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL | 1995年 / 36卷 / 01期
关键词：
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
New rapid and effective procedure for simultaneous purification of human cardiac troponin I and troponin T has been developed. Affinity chromatography on immobilized monoclonal antitroponin I antibody C5 was used for purification of the whole troponin complex with a yield of 120 mg from 100 g of tissue. Isolated troponin I and troponin T (about 20 and 35 mg from 100 g tissue) were obtained by conventionally used ion-exchange chromatography. Antibody C5 recognizes conservative epitope of troponin I, therefore the method is applicable for purification of skeletal and cardiac troponin from a number of different animal species.
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页码：195 / 202
页数：8
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