APPLICATION OF AN ELECTROCHEMICAL DETECTOR WITH A GRAPHITE ELECTRODE TO LIQUID-CHROMATOGRAPHIC DETERMINATION OF PENICILLAMINE AND CAPTOPRIL IN BIOLOGICAL SAMPLES

被引：27
|
作者：
WAKABAYASHI, H [1 ]
YAMATO, S [1 ]
NAKAJIMA, M [1 ]
SHIMADA, K [1 ]
机构：
[1] NIIGATA COLL PHARM,DEPT ANALYT CHEM,NIIGATA 95021,JAPAN
来源：
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1994年 / 12卷 / 09期
关键词：
PENICILLAMINE; CAPTOPRIL; SERUM AND TISSUES; ELECTROCHEMICAL DETECTION; REVERSED-PHASE HPLC; GRAPHITE ELECTRODE;
D O I：
10.1016/0731-7085(94)00072-7
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A high-performance liquid chromatographic determination of penicillamine and captopril in rat serum, liver and kidney samples is described. An electrochemical detector with a graphite working electrode at a potential of +0.9 V vs the Ag/AgCl reference electrode is used for the detection system. Linear responses of the peak height to the amount of samples injected were obtained in a range of 0.1-500 ng on-column and 0.5-500 ng on-column for penicillamine and captopril with correlation coefficients of 0.997 and 0.995, respectively. Detection limits at a signal-to-noise ratio of 3 were 20 and 300 pg for penicillamine and captopril, respectively. The graphite electrode has a long lifetime of about 4 months with continuous use, even with the high voltage supplied. The analytical application of this method to the determination of penicillamine and captopril in biological samples was successful.
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页码：1147 / 1152
页数：6
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