CHARACTERIZATION OF A DNA PROBE FOR DETECTION OF MYCOBACTERIUM-TUBERCULOSIS COMPLEX IN CLINICAL-SAMPLES BY POLYMERASE CHAIN-REACTION

被引：31
|
作者：
ALTAMIRANO, M [1 ]
KELLY, MT [1 ]
WONG, A [1 ]
BESSUILLE, ET [1 ]
BLACK, WA [1 ]
SMITH, JA [1 ]
机构：
[1] BRITISH COLUMBIA CTR DIS CONTROL, VANCOUVER V5Z 1L8, BC, CANADA
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 1992年 / 30卷 / 08期
关键词：
D O I：
10.1128/JCM.30.8.2173-2176.1992
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We cloned and sequenced a DNA fragment from Mycobacterium tuberculosis for use in the identification of members of the M. tuberculosis complex. The DNA probe for culture confirmation had a sensitivity and a specificity of 100%. By using primers developed from this probe, the polymerase chain reaction detected 20 mycobacteria by ethidium bromide staining. This polymerase chain reaction system demonstrated 98% sensitivity and 100% specificity for detection of the M. tuberculosis complex in 200 sputum specimens.
引用
收藏
页码：2173 / 2176
页数：4
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