INDUCTION OF IMMEDIATE-EARLY GENES BY CYCLIC-AMP IN PRIMARY CULTURES OF NEURONS FROM RAT CEREBRAL-CORTEX

被引：44
|
作者：
VACCARINO, FM [1 ]
HAYWARD, MD [1 ]
LE, HN [1 ]
HARTIGAN, DJ [1 ]
DUMAN, RS [1 ]
NESTLER, EJ [1 ]
机构：
[1] YALE UNIV, SCH MED, DEPT PSYCHIAT, NEW HAVEN, CT 06510 USA
来源：
MOLECULAR BRAIN RESEARCH | 1993年 / 19卷 / 1-2期
关键词：
C-FOS; C-JUN; NGFI-A; CEREBRAL CORTEX; PROTOONCOGENE; VASOACTIVE INTESTINAL PEPTIDE;
D O I：
10.1016/0169-328X(93)90151-E
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
In this paper, we tested whether physiological activators of the cAMP second messenger pathway in primary cultures of neurons from rat cerebral cortex directly induce c-fos and other immediate early gene (IEG) transcription factors. We have found that brief (30 s to 2 min) stimulation of neurons with vasoactive intestinal peptide (VIP) and SKF-38393, a D1-dopaminergic receptor agonist, potently increased mRNA levels for the IEGs c-fos, jun-B, and NGFI-A, with weaker increases for c-jun. This action was mimicked by forskolin and dibutyryl cAMP. IEG induction by VIP and dibutyryl cAMP was not blocked by excitatory amino acid receptor antagonists or by blockers of dihydropyridine-sensitive calcium channels. Moreover, calcium-free medium did not modify IEG induction by dibutyryl cAMP, suggesting that cAMP can directly regulate IEG expression in differentiated neurons independently of calcium.
引用
收藏
页码：76 / 82
页数：7
相关论文
共 50 条
12345