IDENTIFICATION AND CLONING OF A CYCLIC BETA-(1-]3), BETA-(1-]6)-D-GLUCAN SYNTHESIS LOCUS FROM BRADYRHIZOBIUM-JAPONICUM

被引:7
|
作者
BHAGWAT, AA
TULLY, RE
KEISTER, DL
机构
[1] UNIV MARYLAND,DEPT AGRON,COLL PK,MD 20742
[2] USDA ARS,BELTSVILLE AGR RES CTR,SOYBEAN & ALFALFA RES LAB,BELTSVILLE,MD 20705
关键词
BETA-GLUCANS; BRADYRHIZOBIUM-JAPONICUM; RHIZOBIUM-MELILOTI; NODULATION;
D O I
10.1016/0378-1097(93)90510-9
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A cosmid clone complementing a cyclic beta-(1 --> 2)-glucan biosynthesis (ndvB) mutant of Rhizobium meliloti was isolated from a Bradyrhizobium japonicum gene library. This clone specified synthesis of beta-(1 --> 3),beta-(1 --> 6)-linked glucans in R. meliloti. The complemented strain was osmotically tolerant and symbiotically competent on alfalfa. Thus, beta-(1 --> 3),beta-(1 --> 6)-glucans can substitute functionally for beta-(1 --> 2)-glucans in R. meliloti.
引用
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页码:139 / 144
页数:6
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