DETECTION OF ANTIBODY TO HEPATITIS-C E2/NS1 PROTEIN IN PATIENTS WITH TYPE-C HEPATITIS

被引：5
|
作者：
YOKOSUKA, O [1 ]
ITO, Y [1 ]
IMAZEKI, F [1 ]
OHTO, M [1 ]
OMATA, M [1 ]
机构：
[1] CHIBA UNIV,SCH MED,DEPT MED 1,CHUO KU,CHIBA 260,JAPAN
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 189卷 / 01期
关键词：
D O I：
10.1016/0006-291X(92)91595-H
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Putative E2/NS1 sequence of hepatitis C virus was expressed in E. coli as a fusion protein with maltose binding protein. Approximately 80 kDa protein was obtained containing 38 kDa E2/NS1 protein. The antibody to this protein was detectable in the same serum from which the sequence was amplified. It was also detectable in none of 7 acute hepatitis, in 2 of 12 chronic persistent hepatitis, in 3 of 25 chronic active hepatitis, and in 2 of 4 cirrhosis. It was detectable in none of 10 normal subjects. In 3 cases who were positive for the antibody before the interferon treatment, it became undetectable after the treatment. Thus,it seems that the antibody is not a neutralizing antibody and is related to active viral replication. © 1992.
引用
收藏
页码：565 / 571
页数：7
相关论文
共 50 条
  • [41] LEVELS OF HEPATITIS-C VIRUS-RNA AND LIVER HISTOLOGY IN CHRONIC TYPE-C HEPATITIS
    GORDON, SC
    KODALI, VP
    SILVERMAN, AL
    DMUCHOWSKI, CF
    URDEA, MS
    CHAN, CS
    WILBER, JC
    AMERICAN JOURNAL OF GASTROENTEROLOGY, 1994, 89 (09): : 1458 - 1461
  • [42] MIXED INFECTIONS OF HEPATITIS-C VIRUS AS A FACTOR IN ACUTE EXACERBATIONS OF CHRONIC TYPE-C HEPATITIS
    KAO, JH
    CHEN, PJ
    LAI, MY
    YANG, PM
    SHEU, JC
    WANG, TH
    CHEN, DS
    JOURNAL OF INFECTIOUS DISEASES, 1994, 170 (05): : 1128 - 1133
  • [43] Comparison of the rate of sequence variation in the hypervariable region of E2/NS1 region of hepatitis C virus in normal and hypogammaglobulinemic patients
    Booth, JCL
    Kumar, U
    Webster, D
    Monjardino, J
    Thomas, HC
    HEPATOLOGY, 1998, 27 (01) : 223 - 227
  • [44] Analysis of the hypervariable region of hepatitis C virus E2/NS1 gene in an infant infected by blood transfusion
    Sugiyama, K
    Goto, K
    Miyake, Y
    Ando, T
    Oda, T
    Maniwa, H
    Li, R
    Kawabe, Y
    Wada, Y
    PEDIATRIC RESEARCH, 1997, 42 (02) : 247 - 250
  • [45] Analysis of the Hypervariable Region of Hepatitis C Virus E2/NS1 Gene in an Infant Infected by Blood Transfusion
    Kohachiro Sugiyama
    Kenji Goto
    Yoshishige Miyake
    Toshihiro Ando
    Takaya Oda
    Hiroko Maniwa
    Rou Li
    Yoshikazu Kawabe
    Yoshiro Wada
    Pediatric Research, 1997, 42 : 247 - 250
  • [46] Degree and distribution of variability in the 5′ untranslated, E1, E2/NS1 and NS5 regions of the hepatitis C virus (HCV)
    Vizmanos, JL
    Gonzalez-Navarro, CJ
    Novo, FJ
    Civeira, MP
    Prieto, J
    Gullon, A
    Garcia-Delgado, M
    JOURNAL OF VIRAL HEPATITIS, 1998, 5 (04) : 227 - 240
  • [47] THE NS2 PROTEIN OF HEPATITIS-C VIRUS IS A TRANSMEMBRANE POLYPEPTIDE
    SANTOLINI, E
    PACINI, L
    FIPALDINI, C
    MIGLIACCIO, G
    LAMONICA, N
    JOURNAL OF VIROLOGY, 1995, 69 (12) : 7461 - 7471
  • [48] IGM ANTIBODY TO HEPATITIS-C VIRUS IN ACUTE AND CHRONIC HEPATITIS-C
    QUIROGA, JA
    CAMPILLO, ML
    CATILLO, I
    BARTOLOME, J
    PORRES, JC
    CARRENO, V
    HEPATOLOGY, 1991, 14 (01) : 38 - 43
  • [49] Expression of glycoprotein hepatitis C virus in mammalian cell and application of Purified proton for detection of antibody against E2 in hepatitis C patients
    朱朝敏
    吴朝栋
    陶其敏
    冯百芳
    常锦红
    中华肝脏病杂志, 1999, (04) : 214 - 216
  • [50] HEPATITIS-C CORE ANTIBODY DETECTION IN ACUTE HEPATITIS AND CIRRHOSIS PATIENTS FROM TUNISIA
    COURSAGET, P
    SIMPSON, B
    ELGOULLI, N
    BENKHELIFA, H
    KASTALLY, R
    PATHOLOGIE BIOLOGIE, 1992, 40 (06): : 646 - 648
12345