THE POLYMERASE CHAIN-REACTION

被引:0
|
作者
HILDERINK, PF [1 ]
WAGENAAR, JA [1 ]
VANDERGIESSEN, JWB [1 ]
VANDERZEIJST, BAM [1 ]
机构
[1] FAC DIERGENEESKUNDE UTRECHT,VAKGRP INFECTIEZIEKTEN & IMMUNOL,3508 TD UTRECHT,NETHERLANDS
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中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
The polymerase chain reaction (PCR) is a technique that enables us to prepare in a test tube billions of copies of a specific piece of DNA. The principles and practical aspects of the PCR are exposed. Within veterinary medicine the most important applications are expected to be i) the diagnostics of infectious diseases and ii) the detection of genetic disorders or desired genetic traits. The PCR can be carried out on a multitude of samples ranging from mummies to paraffine-embedded tissue specimens. However, inhibitors present in e.g. serum, urine and faeces have to be removed.
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页码:1111 / 1117
页数:7
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