ISOLATION, SCREENING AND CHARACTERIZATION OF PROMISINGA-AMYLASE PRODUCING BACTERIA FROM SEWAGE ENRICHED SOIL

被引：0
|
作者：
Pokhrel, Bharat [1 ]
Wanjare, Priyesh [1 ]
Singh, Suman [1 ]
Purushotham, B. [2 ]
Swamy, Kumara M. [1 ]
机构：
[1] Padmashree Inst Management & Sci, Dept Biotechnol, Bangalore 560060, Karnataka, India
[2] East West Coll Sci, Bangalore 560091, Karnataka, India
来源：
INTERNATIONAL JOURNAL OF ADVANCED BIOTECHNOLOGY AND RESEARCH | 2013年 / 4卷 / 02期
关键词：
alpha-amylase; starch hydrolysis; cultural characterization; amylase activity; pH; temperature;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Among different types of enzymes obtained from microbial sources, amylases are the most widely used in industries. In the present study, bacteria were isolated from sewage soil and screened for the production of alpha-amylase. Among four bacterial isolates, one isolate produced maximum zone of starch hydrolysis. The bacterial isolate was identified as Bacillus sp. and was later used for further characterization. Maximum yield of amylase was obtained after 48h of incubation. The optimum pH for enzyme activity was found to be at pH 7 and the optimum temperature for the activity was found to be at 35 degrees C.
引用
收藏
页码：286 / 290
页数：5
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