TRANSFORMING GROWTH FACTOR-BETA-1 IS A POTENT SURVIVAL FACTOR FOR RAT EMBRYO MOTONEURONS IN CULTURE

被引：132
|
作者：
MARTINOU, JC [1 ]
LEVANTHAI, A [1 ]
VALETTE, A [1 ]
WEBER, MJ [1 ]
机构：
[1] CNRS, PHARMACOL & TOXICOL FONDAMENTALES LAB, F-31077 TOULOUSE, FRANCE
来源：
DEVELOPMENTAL BRAIN RESEARCH | 1990年 / 52卷 / 1-2期
关键词：
Cell sorting; Choline acetyltransferase; Motoneuron; Retrograde labelling; Survival factor; Transforming growth factor;
D O I：
10.1016/0165-3806(90)90233-O
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
We have studied the effects of transforming growth factor β1 (TGFβ1) on the survival of embryonic motoneurons in culture. For this purpose, E14 rat embryo motoneurons were purified to more than 90% homogeneity by cell sorting and cultured at low density on monolayers of cortex astrocytes. Subnanomolar concentrations of TGFβ1 (40-500 pM) increased the survival of motoneurons 2-fold after 9-11 days in culture. The increase in choline acetyltransferase (ChAT) activity per culture caused by TGFβ1 was attributable to its effects on survival. Comparable results were found with motoneurons cultured on lysed astrocytes, suggesting that the effects of the factor are not mediated by non-neuronal cells, but that motoneurons are a target for TGFβ1. © 1990.
引用
收藏
页码：175 / 181
页数：7
相关论文
共 50 条
  • [21] COMPLEX REGULATION OF TRANSFORMING GROWTH FACTOR-BETA-1, FACTOR-BETA-2, AND FACTOR-BETA-3 MESSENGER-RNA EXPRESSION IN MOUSE FIBROBLASTS AND KERATINOCYTES BY TRANSFORMING GROWTH FACTOR-BETA-1 AND FACTOR-BETA-2
    BASCOM, CC
    WOLFSHOHL, JR
    COFFEY, RJ
    MADISEN, L
    WEBB, NR
    PURCHIO, AR
    DERYNCK, R
    MOSES, HL
    MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (12) : 5508 - 5515
  • [22] TRANSFORMING GROWTH FACTOR-BETA-1 (TGF-BETA-1) EXPRESSION AND REGULATION IN RAT CORTICAL ASTROCYTES
    DACUNHA, A
    VITKOVIC, L
    JOURNAL OF NEUROIMMUNOLOGY, 1992, 36 (2-3) : 157 - 169
  • [23] CELLULAR-ORIGIN AND DISTRIBUTION OF TRANSFORMING GROWTH FACTOR-BETA-1 IN THE NORMAL RAT MYOCARDIUM
    EGHBALI, M
    CELL AND TISSUE RESEARCH, 1989, 256 (03) : 553 - 558
  • [24] TRANSFORMING GROWTH FACTOR-BETA-1 EXPRESSION DURING PLACENTAL DEVELOPMENT
    DUNGY, LJ
    SIDDIQI, TA
    KHAN, S
    AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 1991, 165 (04) : 853 - 857
  • [25] BIPHASIC EFFECT OF TRANSFORMING GROWTH FACTOR-BETA-1 ON INVITRO ANGIOGENESIS
    PEPPER, MS
    VASSALLI, JD
    ORCI, L
    MONTESANO, R
    EXPERIMENTAL CELL RESEARCH, 1993, 204 (02) : 356 - 363
  • [26] TEMPORAL AND SPATIAL PATTERNS OF TRANSFORMING GROWTH FACTOR-BETA-1 EXPRESSION IN DEVELOPING RAT MOLARS
    DSOUZA, RN
    HAPPONEN, RP
    RITTER, NM
    BUTLER, WT
    ARCHIVES OF ORAL BIOLOGY, 1990, 35 (12) : 957 - 965
  • [27] POSTTRANSCRIPTIONAL REGULATION OF THE HUMAN TRANSFORMING GROWTH FACTOR-BETA-1 GENE
    KIM, SJ
    PARK, K
    KOELLER, D
    KIM, KY
    WAKEFIELD, LM
    SPORN, MB
    ROBERTS, AB
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1992, 267 (19) : 13702 - 13707
  • [28] CELL LINEAGE SPECIFICITY OF EXPRESSION OF THE MURINE TRANSFORMING GROWTH FACTOR-BETA-3 AND TRANSFORMING GROWTH FACTOR-BETA-1 GENES
    WATRIN, F
    SCOTTO, L
    ASSOIAN, RK
    WOLGEMUTH, DJ
    CELL GROWTH & DIFFERENTIATION, 1991, 2 (02): : 77 - 83
  • [29] Effects of transforming growth factor-beta-1 neutralizing antibody and transforming growth factor-beta-3 on the development of tracheal stenosis
    Elicora, Aykut
    Ozcelikay, Goksu
    Sezer, Huzeyin Fatih
    Liman, Serife Tuba
    Yildiz, Kursat
    Topcu, Salih
    Arica, Betul
    TURK GOGUS KALP DAMAR CERRAHISI DERGISI-TURKISH JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY, 2017, 25 (03): : 433 - 438
  • [30] TRANSFORMING GROWTH FACTOR-BETA-1 - AN AUTOCRINE REGULATOR OF ADRENOCORTICAL STEROIDOGENESIS
    FEIGE, JJ
    COCHET, C
    SAVONA, C
    SHI, DL
    KERAMIDAS, M
    DEFAYE, G
    CHAMBAZ, EM
    ENDOCRINE RESEARCH, 1991, 17 (1-2) : 267 - 279
12345