ANTITHROMBIN-III ASSAY WITHOUT INFLUENCE OF HEPARIN COFACTOR-II

被引：2
|
作者：
ENOMOTO, M [1 ]
TANIMIZU, I [1 ]
SAKURAGAWA, N [1 ]
机构：
[1] TOYAMA MED & PHARMACEUT UNIV,DEPT CLIN LAB MED,SUGITANI,TOYAMA 93001,JAPAN
来源：
THROMBOSIS RESEARCH | 1990年 / 57卷 / 05期
关键词：
Antithrombin-III; Antithrombin-III deficient plasma; Clotting time method; Heparin cofactor II;
D O I：
10.1016/0049-3848(90)90030-G
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
A new biological method for the assay of plasma antithrombin-III (AT-III) activity was developed without influence from heparin cofactor II (HC-II). AT-III deficient plasma is used as a substrate and diluted prothrombin time reagent as a reaction trigger for the specific assay of AT-III. The AT-III deficient plasma is prepared by passage of plasma through a heparin-agarose column. In the presence of heparin, AT-III in the sample shows concentration dependent anticoagulant activity and calibration curve is linear on semi-logarithmic graph paper. The results of reproducibility, recoveries and correlation studies with a chromogenic assay indicate that this biological method is reliable and suitable for routine use in clinical laboratories. Influence of HC-II is minimal. The method provides several advantages over those of chromogenic substrate and fibrinogen. © 1990.
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页码：729 / 736
页数：8
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