COMPARATIVE STUDY OF ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY OF PLANT EXTRACT AMLA [Phyllanthus emblica L.] TULSI [Ocimum tenuiflorum L.] NEEM [Azadirachta indica A. JUSS]

Methanolic extracts of dried leaves of Ocimum tenuiflora, Azadirachta indica, Phyllanthus emblica were used for the comparative study of antibacterial and antioxidant activity. The antioxidant activity of these extracts were determined by DPPH [1, 1- Diphenyl- 2-picryl hydrazyl] assay. It was found that fraction IV of Tulsi shows more antioxidant activity when compared to Neem and Amla [T>N>A]. These extracts were further tested for antibacterial activity by spread plate method against Bacillus subtilis, Streptococcus aureus. It was found that Gram negative bacteria were largely inhibited by the fraction III of Tulsi than that of Neem and Amla against reference antibacterial drug like Tetracyclin. The zone of inhibition was measured which shows that fraction III of Tulsi is having high antibacterial activity when compared to Neem and Amla.