NEW STRATEGIES TO ESTABLISH HUMAN MONOCLONAL-ANTIBODIES

被引:3
|
作者
KUDO, T
SAEKI, H
SAIJYO, S
SATO, N
NUMASAKI, M
TACHIBANA, T
机构
[1] TOHOKU UNIV, DEPT SURG, SENDAI, MIYAGI 980, JAPAN
[2] TOHOKU UNIV, DEPT INTERNAL MED, SENDAI, MIYAGI 980, JAPAN
[3] TOHOKU UNIV, DEPT IMMUNOL, TB & CANC RES INST, SENDAI, MIYAGI 980, JAPAN
来源
TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE | 1992年 / 168卷 / 02期
关键词
HUMAN MONOCLONAL ANTIBODY; SCID-HU MOUSE; GIT MEDIUM; CELL FUSION;
D O I
10.1620/tjem.168.323
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
In order to establish human monoclonal antibodies to any sort of antigens efficiently, we have made following two approaches. Our first approach is to improve cell fusion frequency. By improving our previous method for production of human hybridomas, we obtained higher frequency (1/700 vs. 1/5500) compared with our previous method by adding irradiated myeloma cells to culture of fusion cells and modifying the selective medium. Our second approach is to use a SCID-hu mouse for immunization. Since the injection of human PBL can result in the stable long-term reconstitution of a human immune system in SCID mouse, we tried to immune SCID-hu mouse with KLH. In the serum of immunized SCID-hu mouse, we obtained human IgG antibodies to KLH. Additionally, we succeeded in establishing human B lymphoblastoid cell lines which produced antibodies specific to KLH. These methods will open new prospects for the detection and therapy of cancer.
引用
收藏
页码:323 / 327
页数:5
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