APPRAISAL OF FLUORESCENCE IN-SITU HYBRIDIZATION (FISH) TECHNIQUES IN PRENATAL-DIAGNOSIS

被引：4
|
作者：
GRAO, P [1 ]
GUITART, M [1 ]
COLL, MD [1 ]
EGOZCUE, J [1 ]
机构：
[1] HOSP SABADELL,DEPT GENET,BARCELONA,SPAIN
来源：
EARLY HUMAN DEVELOPMENT | 1993年 / 33卷 / 02期
关键词：
IN-SITU HYBRIDIZATION; AMNIOCYTES; CHORIONIC VILLI; PROBES;
D O I：
10.1016/0378-3782(93)90205-9
中图分类号：
R71 [妇产科学];
学科分类号：
100211 ;
摘要：
The reliability of FISH was appraised using probes for the X and Y chromosome and for chromosomes 12 and 18 in prenatal and adult interphase nuclei. Detection of a single hybridization spot proved to be quite reliable (80-92% positive nuclei). Detection of two hybridization spots was more difficult; percentages of nuclei showing two signals varied between 62-72%. The percentages of nuclei with the correct number of spots was higher in the metaphases occasionally found. Thus, FISH may complement but not replace cytogenetic analysis. For sex determination and for the detection of mosaicism, we suggest the use of two different probes in separate regions of the same preparation.
引用
收藏
页码：101 / 108
页数：8
相关论文
共 50 条
  • [31] FLUORESCENCE IN SITU HYBRIDIZATION (FISH) ANALYSIS FOR THE DIAGNOSIS OF HCC
    Lorber, J.
    Kreipe, H.
    Schlegelberger, B.
    Manns, M. P.
    Skawran, B.
    Greten, T. F.
    JOURNAL OF HEPATOLOGY, 2010, 52 : S225 - S225
  • [32] Fluorescence in situ hybridization (FISH) in the diagnosis of urine samples
    Nordberg, ML
    Veeramachaneni, R
    Martin, S
    Herrera, GA
    Turbat-Herrera, EA
    CYTOMETRY, 2001, 46 (05): : 321 - 321
  • [33] Application of fluorescence in situ hybridization (FISH) techniques to fish genetics: A review
    Phillips, RB
    Reed, KM
    AQUACULTURE, 1996, 140 (03) : 197 - 216
  • [34] Prenatal diagnosis of supernumerary marker chromosome using traditional banding and fluorescence in situ hybridization techniques
    Bagci, G
    Acar, H
    Alper, Ö
    Tomruk, H
    Lüleci, G
    CYTOGENETICS AND CELL GENETICS, 1999, 85 (1-2): : 138 - 138
  • [35] FLUORESCENCE IN-SITU HYBRIDIZATION
    PRICE, CM
    BLOOD REVIEWS, 1993, 7 (02) : 127 - 134
  • [36] AMERICAN-COLLEGE-OF-MEDICAL-GENETICS - PRENATAL INTERPHASE FLUORESCENCE IN-SITU HYBRIDIZATION (FISH) POLICY STATEMENT
    不详
    AMERICAN JOURNAL OF HUMAN GENETICS, 1993, 53 (02) : 526 - 527
  • [37] PRENATAL DETECTION OF CRI DU CHAT SYNDROME ON UNCULTURED AMNIOCYTES USING FLUORESCENCE IN-SITU HYBRIDIZATION (FISH)
    PETTENATI, MJ
    HAYWORTH, R
    COX, K
    RAO, PN
    CLINICAL GENETICS, 1994, 45 (01) : 17 - 20
  • [38] FLUORESCENCE IN-SITU HYBRIDIZATION IN THE DIAGNOSIS OF HALF CRYPTIC TRANSLOCATIONS
    NEIDICH, JA
    SCHELLEV, SL
    MILLER, IP
    EVANS, JW
    BANGS, CD
    HSIEH, CL
    PEDIATRIC RESEARCH, 1995, 37 (04) : A151 - A151
  • [39] Interphase fluorescence in-situ hybridization in the diagnosis of bladder cancer
    Skacel, M
    Liou, LS
    Pettay, JD
    Tubbs, RR
    FRONTIERS IN BIOSCIENCE-LANDMARK, 2002, 7 : E27 - E32
  • [40] Application of fluorescence in situ hybridization to chromosome analysis in prenatal diagnosis
    Jankova, R
    Zaharieva, B
    Mazneikova, V
    Dimitrova, V
    Popivanova, P
    Toncheva, D
    EUROPEAN JOURNAL OF HUMAN GENETICS, 2002, 10 : 282 - 282
12345