PRODUCTION OF A MONOCLONAL-ANTIBODY TO HUMAN-LIVER ALKALINE-PHOSPHATASE

A monoclonal antibody to human liver alkaline phosphatase (ALP) was produced by the mouse-hybridoma method using a partially purified enzyme preparation as antigen. The particular hybridoma secreting the antibody was detected by a screening procedure based on the retention of enzyme activity by the enzyme/antibody complex. The antibody cross-reacts strongly with human kidney and bone ALP but not with human placental or intestinal ALP. It also cross-reacts with liver and kidney ALPs from gorilla, chimpanzee and orangutan. It shows no significant reaction, under the conditions used, with liver or kidney ALPs from several lower primates. An antibody affinity column was prepared and shown to be effective for the final stages of liver ALP purification.