TISSUE-SPECIFIC ALTERNATIVE SPLICING OF THE DROSOPHILA-DOPA-DECARBOXYLASE GENE IS AFFECTED BY HEAT-SHOCK

被引:24
|
作者
SHEN, J
BEALL, CJ
HIRSH, J
机构
[1] Department of Biology, University of Virginia, Charlottesville
[2] Biotechnology Center, Ohio State University, Columbus
关键词
D O I
10.1128/MCB.13.8.4549
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Drosophila dopa decarboxylase gene, Ddc, is expressed in the hypoderm and in a small number of cells in the central nervous system (CNS). The unique Ddc primary transcript is alternatively spliced in these two tissues. We investigated whether Ddc splicing in the CNS is a general property of the CNS or a unique property of the cells that normally express Ddc by expressing the Ddc primary transcript ubiquitously under the control of an Hsp70 heat shock promoter. Under basal expression conditions, Ddc splicing shows normal tissue specificity, indicating that the regulation of Ddc splicing in the CNS is tissue specific rather than cell specific. Previous studies have shown that severe beat shock blocks mRNA splicing in cultured Drosophila melanogaster cells. Our results show that splicing of the heat shock-inducible Hsp83 transcript is very resistant to heat shock. In contrast, under either mild or severe heat shock, the splicing specificity of the heat shock-induced Ddc primary transcript is affected, leading to the accumulation of inappropriately high levels of the CNS splice form in non-CNS tissues. The chromosomal Ddc transcript is similarly affected. These results show unexpected heterogeneity in the splicing of individual mRNAs as a response to heat shock and suggest that the Ddc CNS-specific splicing pathway is the default.
引用
收藏
页码:4549 / 4555
页数:7
相关论文
共 50 条
  • [21] A NOVEL, TISSUE-SPECIFIC, DROSOPHILA HOMEOBOX GENE
    BARAD, M
    JACK, T
    CHADWICK, R
    MCGINNIS, W
    EMBO JOURNAL, 1988, 7 (07): : 2151 - 2161
  • [22] Global Patterns of Tissue-Specific Alternative Polyadenylation in Drosophila
    Smibert, Peter
    Miura, Pedro
    Westholm, Jakub O.
    Shenker, Sol
    May, Gemma
    Duff, Michael O.
    Zhang, Dayu
    Eads, Brian D.
    Carlson, Joe
    Brown, James B.
    Eisman, Robert C.
    Andrews, Justen
    Kaufman, Thomas
    Cherbas, Peter
    Celniker, Susan E.
    Graveley, Brenton R.
    Lai, Eric C.
    CELL REPORTS, 2012, 1 (03): : 277 - 289
  • [23] TISA: Tissue-specific alternative splicing in human and mouse genes
    Noh, Seung-Jae
    Lee, Kyooyeol
    Paik, Hyojung
    Hur, Cheol-Goo
    DNA RESEARCH, 2006, 13 (05) : 229 - 243
  • [24] Tissue-specific isoforms of chicken myomesin are generated by alternative splicing
    Bantle, S
    Keller, S
    Haussmann, I
    Auerbach, D
    Perriard, E
    Muhlebach, S
    Perriard, JC
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (32) : 19042 - 19052
  • [25] Tissue-specific splicing factor gene expression signatures
    Grosso, Ana Rita
    Gomes, Anita Q.
    Barbosa-Morais, Nuno L.
    Caldeira, Sandra
    Thorne, Natalie P.
    Grech, Godfrey
    von Lindern, Marieke
    Carmo-Fonseca, Maria
    NUCLEIC ACIDS RESEARCH, 2008, 36 (15) : 4823 - 4832
  • [26] RESPONSE TO HEAT-SHOCK OF GENE-1, A DROSOPHILA-MELANOGASTER SMALL HEAT-SHOCK GENE, IS DEVELOPMENTALLY REGULATED
    VAZQUEZ, J
    MOLECULAR AND GENERAL GENETICS, 1991, 226 (03): : 393 - 400
  • [27] DEVELOPMENT AND TISSUE-SPECIFIC DISTRIBUTION OF MOUSE SMALL HEAT-SHOCK PROTEIN HSP25
    GERNOLD, M
    KNAUF, U
    GAESTEL, M
    STAHL, J
    KLOETZEL, PM
    DEVELOPMENTAL GENETICS, 1993, 14 (02): : 103 - 111
  • [28] The canonical heat shock promoter of the αB-crystallin gene is tissue-specific
    Bhat, SP
    Somasundaram, T
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 1999, 40 (04) : S382 - S382
  • [29] AVIAN CARDIAC TROPOMYOSIN GENE PRODUCES TISSUE-SPECIFIC ISOFORMS THROUGH ALTERNATIVE RNA SPLICING
    FLEENOR, DE
    HICKMAN, KH
    LINDQUESTER, GJ
    DEVLIN, RB
    JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY, 1992, 13 (01) : 55 - 63
  • [30] Alternative splicing of the BSC1 gene generates tissue-specific isoforms in the German cockroach
    Liu, ZQ
    Chung, I
    Dong, K
    INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2001, 31 (6-7) : 703 - 713