REDOX STATUS AND PROTEIN-BINDING OF PLASMA HOMOCYSTEINE AND OTHER AMINOTHIOLS IN PATIENTS WITH HOMOCYSTINURIA

被引:80
|
作者
MANSOOR, MA
UELAND, PM
AARSLAND, A
SVARDAL, AM
机构
[1] Department of Pharmacology and Toxicology, University of Bergen, Haukeland Hospital, Bergen
来源
METABOLISM-CLINICAL AND EXPERIMENTAL | 1993年 / 42卷 / 11期
关键词
D O I
10.1016/0026-0495(93)90202-Y
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Elevations of homocyst(e)ine levels in the blood of patients with homocystinuria may explain the high cardiovascular morbidity. We determined levels of reduced, oxidized, and protein-bound homocyst(e)ine, cyst(e)ine, and cyst(e)inylglycine in plasma from eight patients with homocystinuria. The technique used involved trapping of reduced thiols by collecting blood directly into tubes containing sulfhydryl-reactive reagents. All patients had high levels of homocysteine (range, 1.9 to 91.2 μmol/L), and among the aminothiols investigated, this species showed the most drastic elevation compared with trace levels (<0.4 μmol/L) found in healthy subjects. The ratio between free homocysteine and total homocyst(e)ine (reduced to total ratio) was above normal and positively correlated to the reduced to total ratio for cyst(e)ine, suggesting that an equilibrium exists between these species through sulfhydryl disulfide exchange. The other homocyst(e)ine species (oxidized and protein-bound) were also markedly increased in patients with homocystinuria. Plasma cysteine and cysteinylglycine levels were moderately increased, whereas plasma concentrations of protein-bound cyst(e)ine, protein-bound cyst(e)inylglycine, and free cystine were below normal. Homocysteine in particular and other homocyst(e)ine species are markedly increased in plasma of homocystinurics, and these changes are associated with pronounced alterations in the level and the redox status of other aminothiols. This should be taken into account when considering homocyst(e)ine as an atherogenic agent, and the role of various homocyst(e)ine species in the pathogenesis of homocystinuria. © 1993.
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页码:1481 / 1485
页数:5
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