CHARACTERIZATION OF HEPATOCYTE GROWTH-FACTOR EXPRESSED BY BACULOVIRUS

被引：11
|
作者：
LEE, HS
HUANG, GT
SHEU, JC
CHIOU, LL
LAI, MY
CHEN, DS
LEE, SC
机构：
[1] NATL TAIWAN UNIV,INST MOLEC MED,TAIPEI,TAIWAN
[2] NATL TAIWAN UNIV,DEPT INTERNAL MED,TAIPEI,TAIWAN
[3] NATL TAIWAN UNIV,DEPT EMERGENCY MED,TAIPEI,TAIWAN
[4] NATL TAIWAN UNIV,HEPATITIS RES CTR,TAIPEI,TAIWAN
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 197卷 / 02期
关键词：
D O I：
10.1006/bbrc.1993.2520
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
To express recombinant hepatocyte growth factor (HGF), we cloned the cDNA by polymerase chain reaction. Functional recombinant HGF was expressed in insect cells by a baculovirus expression system. The recombinant HGF is a mixture of a single-chain precursor and a two-chain heterodimer. Under non-reducing conditions, they migrate together as a single band with MW 67 kDa on Western blots. Under reducing conditions, the precursor migrates at 91 kDa, α-chain at 64 kDa and β-chain at 34 kDa. About 25% of the expressed HGF is in the expression medium, which does have scatter activity on HepG2 and MDCK cells and proliferation activity on BN CL.2 cells (a mouse hepatocytes cell line) in a dose-dependent manner. © 1993 Academic Press. All rights reserved.
引用
收藏
页码：591 / 598
页数：8
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