RAPID-DETERMINATION OF ORGANIC-SOLVENT METABOLITES IN URINE BY REVERSED PHASE HPLC

We have developed an improved method for the determination of organic solvent metabolites (HAs), hippuric acid (HA), mandelic acid (MA), o-methylhippuric acid (o-MHA), m-methylhippuric acid (m-MHA), p-methylhippuric acid (p-MHA) and creatinine (CR), in urine by reversed phase HPLC. The separation was carried out with a stainless-steel column (4.0 mm x 200 mm) packed with Wakosil II-5C18-100 and 8% 2-propanol/10 mM phosphate buffer (pH 2.4) containing 6 mM 1-octanesulfonic acid sodium salt as a mobile phase (1.0 ml/min). A UV monitor (210 nm) was used for monitoring. Reproducibility of this method was excellent. Calibration plots between 10 and 1000 ng/injection were linear and passed through the origin of the coordinate system. This method was used for the determination of HAs in urine on volunteers.