RANDOM AMPLIFIED POLYMORPHIC DNA ASSAY IS LESS DISCRIMINANT THAN PULSED-FIELD GEL-ELECTROPHORESIS FOR TYPING STRAINS OF METHICILLIN-RESISTANT STAPHYLOCOCCUS-AUREUS

被引:135
|
作者
SAULNIER, P
BOURNEIX, C
PREVOST, G
ANDREMONT, A
机构
[1] INST GUSTAVE ROUSSY,ECOL MICROBIENNE LAB,F-94805 VILLEJUIF,FRANCE
[2] FAC MED STRASBOURG,INST BACTERIOL,STRASBOURG,FRANCE
[3] FAC PHARM CHATENAY MALABRY,MICROBIOL LAB,F-92290 CHATENAY MALABRY,FRANCE
关键词
D O I
10.1128/JCM.31.4.982-985.1993
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Twenty-six strains of methicillin-resistant Staphylococcus aureus with different pulsed-field gel electrophoresis fingerprints were tested by random amplified polymorphic DNA assay with three primers, resulting in 15 to 20 different random amplified polymorphic DNA fingerprints. By summing the results for the three primers, the number of different fingerprints increased to 25, but two strains could not be differentiated. We conclude that pulsed-field gel electrophoresis remains the best method of typing methicillin-resistant S. aureus strains.
引用
收藏
页码:982 / 985
页数:4
相关论文
共 50 条
  • [41] Molecular typing of methicillin-resistant Staphylococcus aureus by pulsed-field gel electrophoresis:: Comparison of results obtained in a multilaboratory effort using identical protocols and MRSA strains
    Chung, M
    De Lencastre, H
    Matthews, P
    Tomasz, A
    Adamsson, I
    De Sousa, MA
    Camou, T
    Cocuzza, C
    Corso, A
    Couto, I
    Dominguez, A
    Gniadkowski, M
    Goering, R
    Gomes, A
    Kikuchi, K
    Marchese, A
    Mato, R
    Melter, O
    Oliveira, D
    Palacio, R
    Sá-Leao, R
    Sanches, IS
    Song, JH
    Tassios, PT
    Villari, P
    MICROBIAL DRUG RESISTANCE-MECHANISMS EPIDEMIOLOGY AND DISEASE, 2000, 6 (03): : 189 - 198
  • [42] Genotyping of European isolates of methicillin-resistant Staphylococcus aureus by fluorescent amplified-fragment length polymorphism analysis (FAFLP) and pulsed-field gel electrophoresis (PFGE) typing
    Grady, R
    Blanc, D
    Hauser, P
    Stanley, J
    JOURNAL OF MEDICAL MICROBIOLOGY, 2001, 50 (07) : 588 - 593
  • [43] Development of a Canadian standardized protocol for subtyping methicillin-resistant Staphylococcus aureus using pulsed-field gel electrophoresis
    Mulvey, MR
    Chui, L
    Ismail, J
    Louie, L
    Murphy, C
    Chang, N
    Alfa, M
    JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (10) : 3481 - 3485
  • [44] EVALUATION OF INTERHOSPITAL SPREAD OF METHICILLIN-RESISTANT STAPHYLOCOCCUS-AUREUS IN SAO-PAULO, BRAZIL, USING PULSED-FIELD GEL-ELECTROPHORESIS OF CHROMOSOMAL DNA (VOL 15, PG 320, 1994)
    SADER
    INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY, 1994, 15 (08): : 508 - 508
  • [45] Comparison of the DiversiLab Repetitive Element PCR System with spa Typing and Pulsed-Field Gel Electrophoresis for Clonal Characterization of Methicillin-Resistant Staphylococcus aureus
    Babouee, B.
    Frei, R.
    Schultheiss, E.
    Widmer, A. F.
    Goldenberger, D.
    JOURNAL OF CLINICAL MICROBIOLOGY, 2011, 49 (04) : 1549 - 1555
  • [46] Pulsed-field gel electrophoresis (PFGE) as an epidemiological marker for typing of methicillin-resistant Staphylococcus aureus recovered from King Saudi Arabia KSA
    Moussa, I. M.
    Al-Qahtani, A. A.
    Gassem, M. A.
    Ashgan, M. H.
    Ismail, Dalia Kadry
    Ghazy, A., I
    Shibl, A. M.
    AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH, 2011, 5 (12): : 1492 - 1499
  • [47] Pulsed-field gel electrophoresis is more efficient than ribotyping and random amplified polymorphic DNA analysis in discrimination of Pasteurella haemolytica strains
    Kodjo, A
    Villard, L
    Bizet, C
    Martel, JL
    Sanchis, R
    Borges, E
    Gauthier, D
    Maurin, F
    Richard, Y
    JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (02) : 380 - 385
  • [48] Phage typing combined with pulsed-field gel electrophoresis and random amplified polymorphic DNA increases discrimination in the epidemiological analysis of Salmonella enteritidis strains
    Laconcha, I
    Lopez-Molina, N
    Rementeria, A
    Audicana, A
    Perales, I
    Garaizar, J
    INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1998, 40 (1-2) : 27 - 34
  • [49] Comparison of pulsed-field gel electrophoresis and phage typing for discriminating poultry strains of Staphylococcus aureus
    Shimizu, A
    Kawano, J
    Yamamoto, C
    Kakutani, O
    Fujita, M
    AMERICAN JOURNAL OF VETERINARY RESEARCH, 1997, 58 (12) : 1412 - 1416
  • [50] Characterization of isolates of methicillin-resistant Staphylococcus aureus from Hong Kong by phage typing, pulsed-field gel electrophoresis, and fluorescent amplified-fragment length polymorphism analysis
    Ip, M
    Lyon, DJ
    Chio, F
    Enright, MC
    Cheng, AF
    JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (11) : 4980 - 4985