MEDIATORLESS HYDROGEN-PEROXIDE ELECTRODE BASED ON HORSERADISH-PEROXIDASE ENTRAPPED IN POLY(O-PHENYLENEDIAMINE)

被引：79
|
作者：
DENG, Q [1 ]
DONG, SJ [1 ]
机构：
[1] CHINESE ACAD SCI,CHANGCHUN INST APPL CHEM,ELECTROANALYT CHEM LAB,CHANGCHUN 130022,PEOPLES R CHINA
来源：
JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 1994年 / 377卷 / 1-2期
基金：
中国国家自然科学基金;
关键词：
D O I：
10.1016/0022-0728(94)03465-6
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A mediatorless H2O2 sensor based on coelectropolymerization of horse radish peroxidase (HRP) and o-phenylenediamine (o-PD) is described. The electrode responds to H2O2 in a few seconds and gives a current density of 73.3 nA 1 mu mol(-1) cm(-2) at -100 mV (versus Ag\AgCl). A linear calibration curve is obtained over the range 1-130 mu mol l(-1). From kinetic analyses, the response of the biosensor is rate limited by the enzyme kinetics, and the apparent Michaelis-Menten constant K'(m) is 220 mu mol l(-1). The electrode consisting of HRP entrapped in poly(o-phenylenediamine) film shows good stability because there is some interaction between HRP and o-PD in the electropolymerization.
引用
收藏
页码：191 / 195
页数：5
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