PREVALENCE OF EXTENDED SPECTRUM BETA LACTAMASES PRODUCING KLEBSIELLA PNEUMONIAE ISOLATED AT GOVERNMENT MEDICAL COLLEGE, NANDED

PURPOSE:- The purpose of this study was to know the prevalence of Extended Spectrum beta lactamases (ESBL) in Klebsiella pneumoniae isolated from various clinical specimens. METHODS:- A present study was conducted at Dr. Shankarrao Chavan Government Medical College, Nanded between January 2011 - December 2011. A total number of 201 various clinical samples were processed during the study. 91 strains of Klebsiella pneumoniae were isolated. They were studied for ESBL production by screening test, CLSI disc diffusion method & phenotypic confirmation by disc potentiation test. RESULT:- Out of 91 strains, 71 were found positive for ESBL production by screening test. Out of 71 strains 59 were confirmed by disc potentiation test. So out of 91 strains 59 (64.8%) were confirmed as ESBL producers. Among the ESBL producer Klebsiella pneumoniae, 11(18.64%) were sensitive to Cefotaxime, 06(10.16%) to Ceftriaxone & 10(16.94%) to Ceftazidime by routine Kirby Bauer disc diffusion method. All the Klebsiella pneumoniae isolates were sensitive to Imipenem. Resistance against Ampicillin (10ug) is 100%, Ciprofloxacin (5ug) is 93.22%, Gentamicin (10ug) is 88.13%, Tetracycline (30ug) is 72.9% and Amikacin (30ug) is 18.64%. CONCLUSION: Our study shows presence of ESBL producer Klebsiella pneumoniae in clinical specimens and their prevalence is 64.8%. The routine antimicrobial sensitivity test may fail to detect ESBL. Detection of ESBL production should be carried out as a routine in diagnostic laboratories by disc potentiation test as it is a simple and cost effective test. Antibiotics resistance is significantly more prevalent in ESBL positive isolates as compared to ESBL negative.