Early appearance of age-associated deterioration in mitochondrial function of diaphragm and heart in rats treated with doxorubicin

被引:14
|
作者
Yamada, K [1 ]
Sugiyama, S [1 ]
Kosaka, K [1 ]
Hayakawa, M [1 ]
Ozawa, T [1 ]
机构
[1] NAGOYA UNIV, FAC MED, DEPT BIOMED CHEM, SHOWA KU, NAGOYA, AICHI 466, JAPAN
关键词
doxorubicin; diaphragm; heart; aging; mitochondria;
D O I
10.1016/0531-5565(95)00033-X
中图分类号
R592 [老年病学]; C [社会科学总论];
学科分类号
03 ; 0303 ; 100203 ;
摘要
Age-associated deterioration of mitochondrial energy transduction seems to be a major contributory factor to age-related decline in organ function. Free radicals are likely to be involved in the age-related decline in mitochondrial function. This study was designed to elucidate whether or not doxorubicin, a radical generating drug that was administered to 7-week-old rats, affects age-associated mitochondrial functional changes in diaphragm, heart, and liver. Mitochondria from each tissue were prepared from rats aged 7, 13, 20, 28, 35, and 55 weeks, and the activities of four complexes in the mitochondrial energy transduction system were measured enzymatically. In diaphragm mitochondria of the control group, the complex I activity in 28-week-old rats declined to 82% of the activity in rats aged 7 weeks, and the complex IV activity in 55-week-old rats declined to 70% of the activity in rats aged 7 weeks. On the contrary, a significant decrease in the activity of complex I in rats aged 20 weeks (84%) and that of complex IV in rats aged 35 weeks (86%) were observed in the doxorubicin-treated group. In heart mitochondria, age-related changes in activities of complexes I and IV did not appear in rats aged up to 55 weeks, whereas significant decreases in the activities of complexes I (78%) and IV (90%) were observed in rats aged 35 weeks in the doxorubicin group. Age-related changes in liver mitochondria were not found in rats aged up to 55 weeks, and no deleterious effects of doxorubicin were observed in liver mitochondrial function. From these results, the early appearance of aging effects on mitochondrial function was observed in rats treated with doxorubicin particularly in postmitotic cells.
引用
收藏
页码:581 / 593
页数:13
相关论文
共 50 条
  • [32] Cardiosphere-derived Cells From Young Rats Improve Diastolic Function, Exercise Capacity and Age-associated Biomarkers in Senescent Rats
    Grigorian, Lilian
    Fereydooni, Soraya
    Liu, Weixin
    Middleton, Ryan
    Cho, Jae-Hyung
    Valle, Jackelyn
    Echavez, Antonio
    Marban, Eduardo
    CIRCULATION, 2016, 134
  • [33] L-carnitine and α-lipoic acid improve age-associated decline in mitochondrial respiratory chain activity of rat heart muscle
    Sethumadhavan, Savitha
    Chinnakannu, Panneerselvam
    JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES, 2006, 61 (07): : 650 - 659
  • [34] Age-associated decreased activities of mitochondrial electron transport chain complexes in heart and skeletal muscle: role of L-carnitine
    Kumaran, S
    Subathra, M
    Balu, M
    Panneerselvam, C
    CHEMICO-BIOLOGICAL INTERACTIONS, 2004, 148 (1-2) : 11 - 18
  • [35] Depletion of adenine nucleotide translocator protein in heart mitochondria from doxorubicin-treated rats- Relevance for mitochondrial. dysfunction
    Oliveira, PJ
    Wallace, KB
    TOXICOLOGY, 2006, 220 (2-3) : 160 - 168
  • [36] ACE Inhibitor, Lisinopril, Affects Age-Associated Changes in Cardiovascular and Cognitive Function in Female Dahl Salt-Sensitive Rats
    Shearon, Jennifer
    Fenner, Rachel
    Juhasz, Ondrej
    McDevitt, Ross
    Grigorova, Yulia
    Zahariadis, Eleni
    Long, Jeffrey
    Rapp, Peter
    Lakatta, Edward
    Fedorova, Olga
    FASEB JOURNAL, 2021, 35
  • [37] AGE-ASSOCIATED CHANGES OF NITRIC OXIDE-MEDIATED URETHRAL SMOOTH MUSCLE RELAXATION AND EXTERNAL URETHRAL SPHINCTER FUNCTION IN RATS
    Oshiro, Takuma
    Miyazato, Minoru
    Kimura, Ryu
    Ashikari, Asuka
    Saito, Seiichi
    JOURNAL OF UROLOGY, 2018, 199 (04): : E500 - E500
  • [38] Age-Associated Differences in Activation of Akt/GSK-3β Signaling Pathways and Inhibition of Mitochondrial Permeability Transition Pore Opening in the Rat Heart
    Zhu, Jiang
    Rebecchi, Mario J.
    Tan, Minyi
    Glass, Peter S. A.
    Brink, Peter R.
    Liu, Lixin
    JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES, 2010, 65 (06): : 611 - 619
  • [39] Quercetin Improves Postischemic Recovery of Heart Function in Doxorubicin-Treated Rats and Prevents Doxorubicin-Induced Matrix Metalloproteinase-2 Activation and Apoptosis Induction
    Bartekova, Monika
    Simoncikova, Petra
    Fogarassyova, Maria
    Ivanova, Monika
    Okruhlicova, L'udmila
    Tribulova, Narcisa
    Dovinova, Ima
    Barancik, Miroslav
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2015, 16 (04): : 8168 - 8185
  • [40] Late-onset intermittent fasting dietary restriction as a potential intervention to retard age-associated brain function impairments in male rats
    Singh, Rumani
    Lakhanpal, Dinesh
    Kumar, Sushil
    Sharma, Sandeep
    Kataria, Hardeep
    Kaur, Manpreet
    Kaur, Gurcharan
    AGE, 2012, 34 (04) : 917 - 933