AGROBACTERIUM-MEDIATED TRANSFORMATION OF PEANUT (ARACHIS-HYPOGAEA L) EMBRYO AXES AND THE DEVELOPMENT OF TRANSGENIC PLANTS

被引:0
|
作者
MCKENTLY, AH
MOORE, GA
DOOSTDAR, H
NIEDZ, RP
机构
[1] UNIV FLORIDA, INST FOOD & AGR SCI, DEPT HORT SCI, GAINESVILLE, FL 32611 USA
[2] USDA ARS, HORT RES LAB, ORLANDO, FL 32803 USA
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D O I
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中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Transgenic peanut (Arachis hypogaea L.) plants have been produced using an Agrobacterium-mediated transformation system. Zygotic embryo axes from mature seed were cocultured with Agrobacterium tumefaciens strain EHA101 harboring a binary vector that contained the genes for the scorable marker B-glucuronidase (GUS) and the selectable marker neomycin phosphotransferase II. Nine percent of the germinated seedlings were GUS+. Polymerase chain reaction analysis confirmed that GUS+ shoots and T-1 progeny contained T-DNA. Molecular characterization of one primary transformant and its T-1 and T-2 progeny plants established that T-DNA was integrated into the host genome.
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页码:699 / 703
页数:5
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