PRECOLUMN DERIVATIZATION OF RETINOIC ACID FOR LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE AND COULOMETRIC DETECTION

被引：3
|
作者：
ELMANSOURI, S
TOD, M
LECLERCQ, M
PORTHAULT, M
CHALOM, J
机构：
[1] HOP AVICENNE,SERV PHARM,F-93009 BOBIGNY,FRANCE
[2] FAC MED ALEXIS CARREL,INST PASTEUR,INSERM,U331,F-69327 LYON,FRANCE
[3] LAB SCI ANALYT,F-69622 VILLEURBANNE,FRANCE
[4] EUROBIO,LES ULIS,FRANCE
来源：
ANALYTICA CHIMICA ACTA | 1994年 / 293卷 / 03期
关键词：
COULOMETRY; FLUOROMETRY; LIQUID CHROMATOGRAPHY; RETINOIC ACID;
D O I：
10.1016/0003-2670(94)85029-1
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Derivatization of the carboxyl function of retinoic acid by fluorescent or electroactive reagents prior to liquid chromatography was studied. Ferrocenylethylamine was synthesized and could be coupled to retinoic acid. The coupling reaction involved activation by diphenylphosphinyl chloride. The reaction was carried out at ambient temperature in 50 min with a yield of ca. 95%. The derivative can be detected by coulometric reduction (+ 100 mV) after on-line coulometric oxidation (+ 400 mV). The limit of detection was 1 pmol of derivative on-column, injected in a volume of 10 mu l, but the limit of quantification was 10 pmol of retinoic acid.
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页码：245 / 250
页数：6
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