The growth of different strains of Pleurotus spp. on sugar cane agrowastes was evaluated. Three hybrid strains with good production outcomes and yields exceeding 17% were selected. Strain 184 (P. ostreatus x P. pulmonarius) showed the best results. Three spawn materials (wheat grain, millet grain and milled com cob) at different spawning levels were tested and a significant influence was found. The obtained results were best explained in terms of total nitrogen content of the initial mixture (spawn + substrate), suggesting a probable nitrogen limited growth of the mushroom on sugar cane residues. A 10% millet grain spawn was found to be a reasonable compromise. Productive responses decreased with an increase in bag's capacity (8-10-12 kg), even though the same diameter was maintained in order to avoid pronounced temperature profiles. Smaller bag's capacities (8- 1 0 kg) were recommended. It was also shown that the utilization of water hyacinth (Eichhornia crassipes) mixed 50/50 with sugar cane residues as substrate caused a twofold increase of crop responses, confirming the advantages of this substrate supplementation. The obtained results identified sugar cane agrowastes as a feasible substrate for Pleurotus spp. production with yields and biological efficiencies comparable and to some extent better than others reported with conventional lignocellulosic residues such as cereal straw.