PREPARATION OF AMYLOPLASTS FROM SWEET-POTATO CALLUS-CULTURE

被引：0
|
作者：
LU, CH
LEE, PD
SU, JC
机构：
[1] NATL TAIWAN UNIV,DEPT AGR CHEM,TAIPEI 10764,TAIWAN
[2] ACAD SINICA,INST BIOL CHEM,TAIPEI,TAIWAN
来源：
BOTANICAL BULLETIN OF ACADEMIA SINICA | 1995年 / 36卷 / 04期
关键词：
AMYLOPLAST; CALLUS; IPOMOEA BATATAS; LATENCY TEST; STARCH METABOLISM; SWEET POTATO; TISSUE CULTURE;
D O I：
暂无
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Culture conditions for sweet potato callus were manipulated to enhance starch content. Tissue cultures were frozen in liquid nitrogen, pulverized in a ceramic mortar, and ground with a buffer and sea sand. Fractional centrifugation in a discontinuous Percoll gradient enabled separation of amyloplasts in a 40% Percoll layer sandwiched between 20% and 50% layers. A latency test of marker enzymes for amyloplast, and a preliminary feeding experiment with radio-labeled substrates for starch synthesis confirmed that the preparation is suitable for use in the study of starch biosynthesis.
引用
收藏
页码：223 / 228
页数：6
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